Year: 2014 | Month: December | Volume 7 | Issue 4
In vitro evaluation of plant growth regulators on tissue culture bioassay produced by Pseudomonas species
Abstract:
Fluorescent Pseudomonas sp. is emerging as largest and potentially most promising group of PGPR (plant growth promoting rhizobacteria) that are involved in plant growth enhancement. Plant growth regulators viz., auxins, cytokinins and gibberellins help in plant growth and development. In present study, fluorescent Pseudomonas strains isolated from rhizospheric soil from temperate fruit zone of Himachal Pradesh were investigated for plant growth regulator production i.e. auxins, gibberellins and cytokinins in nutrient broth. All the strains tested produced plant growth regulators in concentrations auxins (1.83-21μg/ml), gibberellins (116.1-485.8μg/ml) and cytokinins (45.4-295.4μg/ml). Two strains (An-1-kul and An-13-kul) were selected on the basis of over all PGPR activities for production of growth regulators. Molecular characterization of best selected Pseudomonas strains were done by 16S-rRNA technique. Plant growth regulators produced by best isolates were further studied to observe their effect on growth of callus formation, shoot formation of broccoli and root elongation of cabbage
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